attune flow cytometer channels

dilute samples to be processed quickly at sample input speeds of up to 1 mL/min, Images. Attune NxT Flow Cytometer (Violet 6-Channel Configuration) CYTO 2017 Jolene Bradford, Associate Director, at Thermo Fisher Scientific, presents the Invitrogen Attune NxT Flow Cytometer (violet 6-channel configuration). A flow cytometer equipped with two lasers (e.g., a 488 nm blue laser or 532 nm green laser and a 633-635 nm red laser) capable of distinguishing 575 nm and 660 nm or a flow cytometer equipped with one laser (e.g., 488 nm blue laser) capable of distinguishing 575 nm and 670 nm. Recommended flow rates. Optics. conditions: use an Attune NxT Flow Cytometer with standard 0.5 mm blocking configuration, an Invitrogen Attune NxT 488/10 Filter (Cat. Life Technologies Attune flow cytometers produces FCS 3.0 compliant files. The Invitrogen Attune cytometers can be designed Using the Attune NxT Flow Cytometer for rapid and accurate analysis of nuclear DNA content in plants. Recorded at Attune CytPix Flow Cytometer. Category Feature Attune CytPix Flow Cytometer Attune NxT Flow Cytometer Optics Number of lasers 24 14 Number of detection channels 2 The instrument comes with up to three lasers (blue, red, violet) and comes with 4 to 12 fluorescent channels, amounting to 14 AttuneNxT Flow Cytometer 1 USER GUIDE Attune NxT Acoustic Focusing Cytometer Catalog Number A24858, A24859, A24860, A24861, A24862, A24863, A24864 Publication Number Revision B.0 For Research Use Only. Category Feature Attune CytPix Flow Cytometer Attune NxT Flow Cytometer Optics Number of lasers 24 14 Number of detection channels 2 Attune NxT Acoustic. The great advantage of flow cytometry is that it allows for the simultaneous detection of several markers on a single cell at the very same time. Features of Attune Flow Cytometers. Attune NxT Flow Cytometer. Cell mor-phology and shape were analyzed based on SSC-A/FSC-A The Attune NxT Flow Cytometer results showed equal or better results even at the highest flow rates. Attune nxt flow cytometer price. Product Manager Flow Cytometry Instrument Systems-Imaging CytometryPosition Objective: The Global Product Manager for Flow Cytometry Instrument Imaging Cytometry is responsible for launching New Product Introductions for Imaging Cytometry. The systems attain sample throughput rates of 1,000 L/min, ten times faster than traditional cytometers without compromising data integrity. Attune Cytometric Software makes the system easy to use with an optional module for 21 CFR part 11 compliance. The Attune NxT Flow Cytometer was developed with the goal of removing barriers associated with flow cytometry and enabling new research. This includes bringing the power of flow cytometry to the analysis of more sample types, including cancer cells. The Invitrogen. The Attune NxT Flow Cytometer is designed for sensitive, rapid, and accurate cell analysis for applications including immunophenotyping and T cell analysis, Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user Development of advanced flow cytometers has driven the necessity for a broader variety of fluorochromes with unique spectral properties to maximize multiplexing capabilities. The AttuneNxT Acoustic Focusing Cytometer has a range of flow rates from 12.5 L/minute to 1000 L/minute. At low sample rates (e.g., 12.5 L/minute and The BD FACSLyric is fast, simple to use, and automation friendly. Flow cytometry data acquisition and analysis were performed by Attune Flow Cytometry Software (Thermo Fisher Scientific). 1:55 PM 2:05 PM: Jennifer Jakubowski. Table 1. They will work with research and development, operations and business counterparts to develop and execute MGPP strategy for No. Plant samples for fl ow cytometry are typically prepared from a small amount of tissue The 2020 Invitrogen Attune NxT Flow Cytometer with acoustic focusing technology is now available with 6 fluorescence detectors off the violet laser, allowing measurement of up to 11 fluorescence parameters with a 2-laser instrument, 12 fluorescence parameters with a 3-laser instrument, or 14 fluorescence parameters with a 4-laser instrument (Table 1). 1 In the past 10 years, flow cytometric immunophenotyping has maintained its position as an indispensable diagnostic tool. Electrical requirements: 100240 VAC, 50/60 Hz, Table 1. The Attune NxT Flow Cytometer Thermo Fisher Scientific certifies that the Attune Flow Cytometers conform to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune Flow Cytometers are Class I laser products per Center for Devices and Radiological Health (CDRH) regulations and EN/IEC 60825. Flow Cytometry Support Center. Attune NxT Flow Cytometer System. Relative background level of detection channel. Partial list of compatible flow cytometers: Flow Cytometer Reporter Jolene Bradford, Associate Director, at Thermo Fisher Scientific, presents the Invitrogen Attune NxT Flow Cytometer (violet 6-channel configuration). 24. Number of lasers. 100083194), and Invitrogen Attune The Attune NxT cytometer showed equal or better results even at the highest flow rates. Register an Attune NxT Flow Cytometer with the Thermo Fisher Cloud to view performance test data on the cloud. The Attune NxT Flow Cytometer is ideally suited for the detection of multiple FPs and fluorescently labeled antibodies (separately or in combination), with configurations allowing up It Is Equipped With Three Lasers And Up To 48 Detection Channels For Fluorescence And Up To Two Detection Channels For Scatter (FSC [forward Scatter] And Violet Laser SSC [side Scatter]). Attune NxT Flow Cytometer combines precision with performance in a true benchtop flow cytometer with up to 4 lasers and 16 parameters of detection. Hot Flow: Attune Plumbing for Radioactive Samples. Run samples faster and achieve greater resolution while minimizing sample loss due to clogging. Attune data files have a maximum range of about 1 million channels for both linear and log parameters, so Hard drives: 2 x 2TB SATA 6.0Gb/s, 7200 RPM, 64MB data burst cache; Controller RAID1, integrated. yses, the sheat fluid flow rate was set at 200 l/min. Features of Attune Flow Cytometers. Description. Detectors that measure the output from the 488-nm laser (blue). Activated B cells express CD30, a regulator of apoptosis. Flow Cytometer Reporter Channel Channel Emission Classification Channel Channel Emission Compensation Needed Attune NxT YL1 585/16 RL1 670/14 No* iQue Plus Screener YL1 586/20 Installation requirements. Browse the laser configurations, as well as specifications for optics, fluidics, performance, software, quality & regulatory, and data management specifications. For SSC, FSC, and dsRED2 channels, the area and the height of the global signal were recorded. Browse the laser configurations, as well as specifications for optics, fluidics, performance, software, quality & regulatory, and data management specifications. The conventional cytometer was run using the highest sensitivity setting (~12.5 L/min). The Attune NxT Flow Cytometer was run at 12.5 L/min (B), which is equivalent to the traditional flow cytometer and 500 L/min (C; 40x more sample). The Attune NxT is an easy operating benchtop flow cytometer developed with acoustic-assisted hydrodynamic focusing technology. Attune data files have a maximum range of about 1 million channels for both linear and log parameters, so linear parameters are displayed on a scale that extends to 1M, and log transformed parameters display on a 6-decade scale. The next generation in acoustic focusing cytometry. Attune Flow Cytometers are compact, benchtop cell analyzers that can be configured with up to four spatially separated lasers to flexibly design, run, and analyze panels of up to 14 colors. The Attune NxT cytometer was run at (B) 12.5 L/min, which is equivalent to the traditional flow cytometer and at (C) 500 L/min, which is 40x more sample flow. 74 Attune NxT Acoustic Focusing Cytometer AttuneNxT filters The following replacement filters used in the optics path of the AttuneNxT Acoustic Focusing Cytometer are available separately from Thermo Fisher Scientific. For more information, go to www.thermofisher.comor contact Technical Support (page 88). 102 10 3 104. Clinical Flow Cytometer Beckman Free Pdf Multi-laser, Compact Benchtop Flow Cytometer. It features 4 laser lines (405 nm, 488nm,

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